http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-115198006-A
Outgoing Links
| Predicate | Object |
|---|---|
| assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_f47974102c0abd02e3f47b1d3da6783b |
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-6858 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-6858 |
| filingDate | 2022-07-18-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_7f440c6352b51007533ea2b9669a657f http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_3d42d4c26d3c97fa1a9dd01499c5ff20 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_61e2e06e376e19c3f1f61f437fd2d0c3 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_f73654810508348d6e95f39de98209b9 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_2b8657a280318ed0e24065a7a3bfebaa |
| publicationDate | 2022-10-18-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | CN-115198006-A |
| titleOfInvention | An XNA molecular clamp-multiplex fluorescent PCR method for gene mutation detection |
| abstract | The invention relates to the technical field of microorganisms, in particular to an XNA molecular clamp-multiplex fluorescence PCR method for gene mutation detection; comprising preparing a sample to be tested; extracting DNA and RNA in the sample to be tested, and reversing the RNA, The cRNA is obtained, and then the cRNA and the DNA are mixed and subjected to multiple fluorescent PCR diffusion to obtain a fluorescent sample; the fluorescent sample is detected by a fluorescent PCR amplifier, and the ct value of the fluorescent sample is displayed to determine the detection result. Methods The multiplex fluorescence PCR diffusion technology was introduced, and the real-time PCR system was established by using specific primer and probe technology and three-color fluorescence channel detection mode, which could detect multiple fusion genes and multiple mutant genes at the same time, with good specificity and accuracy. It has high rate, fast and cheap, and solves the problem of time-consuming detection of gene mutation status by existing kits. |
| priorityDate | 2022-07-18-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 108.