http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-115197997-A
Outgoing Links
| Predicate | Object |
|---|---|
| assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_5d3794dcb4f304ad45bd78eaf636b96a |
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-6895 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-6804 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-6806 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-6895 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-6806 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-6804 |
| filingDate | 2022-08-10-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_08b48f71224781e340b5070d81cd45bf http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_276de112ff9fece3d34ecba6109e695c |
| publicationDate | 2022-10-18-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | CN-115197997-A |
| titleOfInvention | A CUT&Tag method applied to plant pollen |
| abstract | The invention discloses a CUT&Tag method applied to plant pollen. The cell nucleus extract in the CUT&Tag method of plant pollen protected by the present invention contains Percoll to remove starch in pollen cells. The method further includes the step of removing polysaccharides and polyphenols in pollen cells by using a buffer solution containing 1,6-hexanediol and glycerol after removing starch. The concentration of the Percoll in the nuclear extract may be 60%. Experiments have proved that the plant pollen CUT&Tag method established in the present invention can effectively remove impurities such as polysaccharides, polyphenols, high starch and the like which are rich in pollen, and can effectively identify the interaction between DNA and proteins in plant pollen. The method of the present invention can also be extended to the study of the interaction between DNA and protein in other tissues of maize or other plants. |
| isCitedBy | http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-116200367-B http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-116200367-A |
| priorityDate | 2022-08-10-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 48.