| abstract |
The invention provides a preparation method of manganese dioxide microspheres modified with dimethylimidazole cobalt. The manganese dioxide microspheres are completely dissolved in a methanol solution of cobalt nitrate, and then a methanol solution of 2-methylimidazole is added, Stir, centrifuge, wash, and dry to obtain dimethylimidazolium-cobalt-modified manganese dioxide microspheres. The material has the advantages of easy synthesis, high specific surface area, high loading capacity, good permeability, short diffusion path, and excellent thermal stability. As a novel enzyme immobilization carrier, acetylcholinesterase was immobilized on dimethylimidazolium-cobalt-modified manganese dioxide microspheres through electrostatic interaction. The invention also provides the application of the immobilized acetylcholinesterase combined with capillary electrophoresis technology to screen the acetylcholinesterase inhibitor from the traditional Chinese medicine Inula. The immobilized acetylcholinesterase of the invention has the advantages of wide pH resistance range, good thermal stability, reusability, strong enzyme activity and the like. |