http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-115197311-A
Outgoing Links
| Predicate | Object |
|---|---|
| assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_dfaf0ce594edf4d2698a5886dbd7cc5f |
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07K14-5434 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C07K1-34 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C07K1-18 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C07K1-14 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C07K14-54 |
| filingDate | 2021-04-09-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_f3833e350dd973b31c6d5846bd00027d http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_ad081e0d83bbdfe85757e92e43ec2f1f http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_da52e15d7594e27ea442392cfdcf8eed http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_0b5a7a57b982df58e459c95bca8f0c30 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_5f626ce330ddcbac60ddbcefcd1a2619 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_242a384546b31a74dbd90a961be9fbf1 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_0d044c100c4435b3b50d48854078edb0 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_4d96be83aef051dad20ca895965528e6 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_d3c1431719bab9ff6a6496fc4efd3d0d |
| publicationDate | 2022-10-18-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | CN-115197311-A |
| titleOfInvention | A kind of purification method of recombinant human interleukin 12 protein |
| abstract | The invention provides a purification method of recombinant human interleukin-12 protein. The method includes: clarifying and filtering the cell culture fluid containing recombinant human interleukin-12 protein; performing compound strong anion exchange chromatography on the cell fluid obtained after the pretreatment, and performing compound weak cation exchange chromatography on the harvested protein sample , the further harvested protein samples are subjected to strong cation exchange chromatography, and finally the purified recombinant human interleukin 12 protein is obtained. The present invention only uses three ion exchange chromatography, with few chromatography steps, and the three purification fillers are all pressure-resistant, high flow rate, and high resolution. The purity is as high as more than 99%, the residual amount of host protein is far less than 0.05%, the content of host DNA is less than 0.1ng/15μg, and the content of bacterial endotoxin meets the requirements, which greatly shortens the production time and cost, and is suitable for industrial production. |
| priorityDate | 2021-04-09-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 51.