http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-115184444-A
Outgoing Links
| Predicate | Object |
|---|---|
| assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_ac31afbea1cbbb03498644721ffb4a62 |
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N27-62 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N27-62 |
| filingDate | 2022-04-14-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_81ebe525fb6b6079e0d57276cf8f5a93 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_a6e2ab985743e023d68d67f01fe10778 |
| publicationDate | 2022-10-14-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | CN-115184444-A |
| titleOfInvention | Device, detection method and application of nano-kit coupled with electrospray ionization mass spectrometry for detecting enzyme activity |
| abstract | The present application provides a device for detecting enzyme activity coupled with a nano-kit coupled with electrospray ionization mass spectrometry, a detection method and an application thereof, so as to measure the enzyme activity in a single living cell. It is specifically disclosed that a nanocapillary tube containing a substrate corresponding to the enzyme to be tested is inserted into a living cell, the cytoplasm is electrochemically extracted, and the substrate in the capillary tube is hydrolyzed; after the mixture is electrosprayed at the tip of the capillary tube, the product Molecular weight differentiation from substrates enables detection of enzymatic activity in single cells. The established detection device and method will provide a specific analysis method for the further study of the enzyme activity in single cells. |
| priorityDate | 2022-04-14-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 134.