| Predicate |
Object |
| assignee |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_8e62810fb19ee19efcb1cec22b9706c0 |
| classificationCPCAdditional |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q2600-16 |
| classificationCPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-6895
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-6851
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-6853 |
| classificationIPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-6853
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-11
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-6895
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-6851 |
| filingDate |
2022-06-21-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_eaade8e4b3bf133e2c88797314c13e3c
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_880f14552e05e4b395571abbab265fcd
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_cbd9b6274832dde92404e37f416ef367
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_3526684765908a9c807a9945488310c2
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_69de9894dae51a37fb728734e2e5969b
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_2884592d55f8e3bd94f5dbf13f8f46d3
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_d5fe776a7a18b8043ea2206c6f6e6113 |
| publicationDate |
2022-10-14-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber |
CN-115181811-A |
| titleOfInvention |
A method for identifying Chinese medicinal materials Ophiopogon japonicus and Ophiopogon japonicus based on MLPA technology |
| abstract |
The invention discloses a method for identifying Chinese medicinal materials Ophiopogon japonicus and Ophiopogon japonicus based on multiple ligation probe amplification technology, comprising the following steps: 1) extracting sample genomic DNA; 2) using genomic DNA as a template, using specificity Amplify the chloroplast gene fragments of Ophiopogon japonicus and Ophiopogon japonicus with primers and purify the amplified product; 3) Hybridize the MLPA probe with the purified product; 4) Use DNA ligase to connect the hybridization product; 5) Use universal primer pair to connect The product was amplified by qPCR; 6) The melting curve signal was collected, and the Tm value was observed. The invention can simultaneously identify Chinese medicinal materials Ophiopogon japonicus and Ophiopogon japonicus in a short time, the method has the advantages of strong specificity and high sensitivity, and has a good application prospect in the field of authenticity identification of Chinese medicinal materials. |
| priorityDate |
2022-06-21-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type |
http://data.epo.org/linked-data/def/patent/Publication |