| abstract |
A method for preparing chitosan oligosaccharide by enzymatic hydrolysis of chitosan relates to the technical field of chitosan oligosaccharide preparation. After chitosan and glacial acetic acid are swollen at a certain temperature, they are degraded into chitosan oligosaccharide liquid under the action of chitosanase, and finally chitosan oligosaccharide powder is obtained by spray drying. On the premise of ensuring the purity, solubility, silkiness and other biological activities of chitosan oligosaccharide, it can effectively reduce the loss of drying and pulverizing materials, shorten the drying time and reduce the production cost, so that the color of the sprayed chitosan oligosaccharide powder is uniform. The present invention degrades chitosan by optimizing the enzyme liquid prepared by the strain, and the prepared chitosan oligosaccharide has high quality. The centrifuged chitosan oligosaccharide liquid is used to exclude other impurities that interfere with the spray effect. Under the conditions of 8%-20% liquid mass concentration of chitosan oligosaccharide and pH 3.0-7.0, the spatial structure will not be destroyed by the high temperature of spray drying, nor will it be denatured into insoluble substances due to high temperature, and it still maintains good purity. and bioactivity such as solubility. |