| abstract |
The invention relates to a nucleic acid aptamer CAGA03 of Helicobacter pylori CagA and its application, belonging to the field of biotechnology. The invention adopts the in vitro SELEX screening technology, utilizes carboxyl magnetic beads as solid-phase medium, takes Helicobacter pylori CagA protein as the target, and obtains nucleic acid suitable for specific binding with Helicobacter pylori CagA by screening from ssDNA library through Helicobacter pylori CagA magnetic beads. Ligand; the nucleic acid aptamer CAGA03 of the present invention can bind to the Helicobacter pylori CagA protein with high affinity and high specificity, and the nucleic acid aptamer CAGA03 is a detection reagent, a purification reagent and a targeting carrier for preparing the Helicobacter pylori CagA protein and antagonists offer new options. |