| abstract |
The invention relates to an integrated antibody detection test strip for primary screening and diagnosis of brucellosis in sheep. The test strip is composed of a preliminary screening test strip (test strip 1) and a diagnosis test strip (test strip 2), which are respectively composed of a PVC bottom plate, a sample pad, a gold label pad, a nitrocellulose membrane and a water-absorbing material. pad composition. Test strip 1 Gold label pad is coated with colloidal gold-labeled Brucella LPS and colloidal gold-labeled mouse-derived anti-Flag-tag monoclonal antibody, the detection line is coated with Brucella monoclonal antibody M4, and the quality control line is coated with sheep Anti-mouse IgG antibody. The test strip 2 gold-labeled pad was coated with colloidal gold-labeled Brucella LPS, the detection line was coated with rabbit anti-goat IgG antibody, and the quality control line was coated with Brucella monoclonal antibody M4. When in use, take the serum to be tested and drop it into the sample hole. According to the results presented by the test strip 1 and the test strip 2, the sample to be tested can be quickly judged as negative, positive or suspicious for brucellosis on the spot. |