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filingDate 2022-06-28-04:00^^<http://www.w3.org/2001/XMLSchema#date>
inventor http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_4aeec55d3264321a251f00eb776a6ab7
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publicationDate 2022-10-11-04:00^^<http://www.w3.org/2001/XMLSchema#date>
publicationNumber CN-115165827-A
titleOfInvention A method for observing cell wall cellulose microfibrils using SIM super-resolution imaging technology
abstract The invention belongs to the field of biotechnology, and provides a method for observing cell wall cellulose microfibrils by using SIM super-resolution imaging technology. In the present invention, the plant protoplast is prepared and the cell wall is regenerated and cultured; after the cells of the regenerated cell wall are stained, an image is obtained by imaging and observation using a SIM microscope. The method of the invention has little phototoxic damage to cells, can observe the cellulose microfibrils for a long time in the living state and the single-cell level, truly reflects the state of the cellulose microfibrils on the surface of the protoplast, and realizes the transformation of the cellulose microfibrils from the protoplasts. The super-resolution imaging of the regenerated primary cell wall cellulose microfibril structure lays the foundation for further qualitative and quantitative research on cellulose microfibrils.
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