http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-115152743-A
Outgoing Links
Predicate | Object |
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assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_ff587db19b24a4cb1ad7f1dbf477cc47 http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_3833ed2e277a6f4631d58a3bbb8c0983 |
classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/A01N1-0221 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/A01N1-0226 |
classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/A01N1-02 |
filingDate | 2022-07-12-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_401db759e8d580ea4e48eee6eba9356f http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_f0aed960a3399ad15c6569c4ea53c576 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_b87f02af184d14c3bcf98d717f259f68 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_f3c65cfc118f2a2e6bfa0a4413075a13 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_78350a50713264b844e938a30d5c3b46 |
publicationDate | 2022-10-11-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
publicationNumber | CN-115152743-A |
titleOfInvention | A kind of preparation method of human umbilical cord mesenchymal stem cell serum-free cryopreservation solution |
abstract | The invention relates to the technical field of stem cell culture, and discloses a method for preparing a serum-free cryopreservation solution of human umbilical cord mesenchymal stem cells, which comprises the following steps: weighing 100 parts by volume of a serum-free medium for mesenchymal stem cells; 10-30 parts of nutritional supplements, 4-10 parts of non-permeable cryoprotectant, 1-3 parts of osmotic cryoprotectant, mixed, sterilized by filtration, and stored to obtain a serum-free cryopreservation solution of human umbilical cord mesenchymal stem cells, Does not contain animal serum and other heterologous proteins, reduces the possibility of human umbilical cord mesenchymal stem cells being contaminated, does not use dimethyl sulfoxide, avoids potential clinical toxicity risks, and maintains human umbilical cord mesenchymal stem cells The biological characteristics and survival rate of the product effectively ensure the differentiation ability of the cells, and the operation is simple and the cryopreservation effect is good. It can be used directly as an auxiliary material and has the characteristics of convenient use. |
priorityDate | 2022-07-12-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 45.