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filingDate 2022-07-12-04:00^^<http://www.w3.org/2001/XMLSchema#date>
inventor http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_401db759e8d580ea4e48eee6eba9356f
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publicationDate 2022-10-11-04:00^^<http://www.w3.org/2001/XMLSchema#date>
publicationNumber CN-115152743-A
titleOfInvention A kind of preparation method of human umbilical cord mesenchymal stem cell serum-free cryopreservation solution
abstract The invention relates to the technical field of stem cell culture, and discloses a method for preparing a serum-free cryopreservation solution of human umbilical cord mesenchymal stem cells, which comprises the following steps: weighing 100 parts by volume of a serum-free medium for mesenchymal stem cells; 10-30 parts of nutritional supplements, 4-10 parts of non-permeable cryoprotectant, 1-3 parts of osmotic cryoprotectant, mixed, sterilized by filtration, and stored to obtain a serum-free cryopreservation solution of human umbilical cord mesenchymal stem cells, Does not contain animal serum and other heterologous proteins, reduces the possibility of human umbilical cord mesenchymal stem cells being contaminated, does not use dimethyl sulfoxide, avoids potential clinical toxicity risks, and maintains human umbilical cord mesenchymal stem cells The biological characteristics and survival rate of the product effectively ensure the differentiation ability of the cells, and the operation is simple and the cryopreservation effect is good. It can be used directly as an auxiliary material and has the characteristics of convenient use.
priorityDate 2022-07-12-04:00^^<http://www.w3.org/2001/XMLSchema#date>
type http://data.epo.org/linked-data/def/patent/Publication

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