| abstract |
Described herein are methods and compositions for expanding dopaminergic neuron progenitor cells, including the use of compositions and media having at least the following components: FGF, SHH signaling agonist, canonical Wnt signaling agonist, and Wnt- C59. The method includes contacting dopaminergic neuron progenitor cells with a medium comprising FGF, an agonist of SHH signaling, a canonical Wnt signaling agonist, and Wnt-C59 to generate an expanded population of dopaminergic neuron progenitor cells. |