http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-115141824-A
Outgoing Links
| Predicate | Object |
|---|---|
| assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_de82f8a051c0a12f46285110ece33b04 |
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-1003 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-10 |
| filingDate | 2022-08-12-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_8589d9e80eff43089a4c13d437314a60 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_2be60e11488e5806b8b411cabef28ec0 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_8099debeaa8ac3b70105e19a68e25e7d http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_8ee5ac62ef9b2fcede9a56bc12f13ac0 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_974374641348ce5799459309bdadb5c2 |
| publicationDate | 2022-10-04-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | CN-115141824-A |
| titleOfInvention | A method for extracting polysaccharide and polyphenolic plant RNA by improved CTAB method |
| abstract | The invention relates to the technical field of plant RNA extraction, in particular to a method for extracting polysaccharide polyphenols plant RNA by an improved CTAB method. The method includes the following steps: grinding the plant tissue sample to powder, adding the improved extract solution to water bath for lysing and extracting, centrifuging to collect the supernatant, adding an equal volume of chloroform to mix evenly and then standing, centrifuging to collect the supernatant, adding 6M LiCl solution to precipitate RNA , collect the precipitate by centrifugation, add 75% anhydrous ethanol to wash the precipitate, then add DNA digestion solution, dissolve RNA in water bath, and finally add DNase inactivation reagent. The polysaccharide and polyphenolic plant RNA extracted by the improved method of the invention can be used for the construction of a full-length transcriptome library, the extraction success rate can be increased from 64% to 82%, and the library construction success rate can be increased from 92% to 98%. |
| priorityDate | 2022-08-12-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 39.