| abstract |
The present invention relates to the biological field, in particular to a serum-free medium and its use and method in culturing muscle stem cells. Based on the total volume of the serum-free medium, the serum-free medium includes the following components: basal culture medium Base, fatty acid-free albumin 1～10mg/mL, ascorbic acid or its derivatives 100～500μg/mL, insulin or its analogs 5～50μg/mL, transferrin 5～50μg/mL, selenite 5 ~50ng/mL, growth factor 5-80ng/mL, neuregulin 0.01-1ng/mL; the serum-free medium can subculture muscle stem cells for a long time for multiple generations, thereby significantly reducing the development and production of muscle stem cells in cell cultured meat. cost issue. |