| abstract |
The invention relates to the technical field of medical detection, in particular to a method for pre-processing a sample for LC-MS/MS determination of gentamicin concentration, comprising: an extraction step: adding an extraction solution to the sample, ultrasonic extraction, centrifugation, collection The supernatant is obtained from the supernatant; purification step: use a PCX cation exchange column to elute the supernatant with ammoniated methanol as the eluent, collect the eluent, dry, redissolve the dried product with a reconstituted solution, and filter to obtain the sample solution; the extraction solution is an aqueous solution containing potassium dihydrogen phosphate, trichloroacetic acid and disodium EDTA; the complex solution is an aqueous solution containing acetic acid and heptafluorobutyric anhydride; the matrix can be effectively removed by this method The interfering substances in the gentamicin and impurity components are effectively separated, thereby improving the sensitivity of the detection as a whole, resulting in significantly improved recovery and significantly lower limit of quantification. |