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filingDate 2022-06-24-04:00^^<http://www.w3.org/2001/XMLSchema#date>
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publicationDate 2022-09-30-04:00^^<http://www.w3.org/2001/XMLSchema#date>
publicationNumber CN-115125182-A
titleOfInvention Construction method and application of squalene synthesis pathway in Bacillus subtilis
abstract The invention discloses a construction method and application of a squalene synthesis pathway in Bacillus subtilis. By using heterologous expression technology and genetic engineering means, the key gene ERG9 for squalene synthesis is connected to the pCE2 plasmid, and P43 is used as the initiator. obtained a recombinant plasmid, amplified and introduced into Bacillus subtilis 168 to form a Bacillus subtilis engineering strain 168-ERG9 capable of synthesizing squalene, and construct a squalene synthesis pathway in Bacillus subtilis, and the transformation The strain has been improved in the synthetic performance of MK‑7.
priorityDate 2022-06-24-04:00^^<http://www.w3.org/2001/XMLSchema#date>
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