| abstract |
The invention discloses an HPLC-UV detection method for the purity of a disodium sulfosuccinate intermediate. Taking an appropriate amount of a disodium sulfosuccinate intermediate, using ACN as a diluent to dissolve it, the concentration is about 1 mg/mL. 10 μL of the sample solution was injected into a liquid chromatograph to detect the purity of the main components and related substances; this method adopts a C8 chromatographic column with good retention of moderately polar compounds, an ammonium acetate aqueous solution/acetonitrile reversed-phase buffer salt chromatographic system, It can enhance the retention of the target compound; for separation by gradient elution, the purity of the disodium sulfosuccinate intermediate can be quickly detected, the main component and its related substances can be effectively separated, and the reaction of the disodium sulfosuccinate intermediate can be monitored. |