http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-115109775-A

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filingDate 2022-04-27-04:00^^<http://www.w3.org/2001/XMLSchema#date>
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publicationDate 2022-09-27-04:00^^<http://www.w3.org/2001/XMLSchema#date>
publicationNumber CN-115109775-A
titleOfInvention Polydeoxyribonucleotide and preparation method thereof
abstract The present application provides a method for preparing polydeoxyribonucleotides, the preparation method comprising the following steps: obtaining salmonid genomic DNA, using multiple replacement to amplify the salmonid genomic DNA to prepare an amplified salmonid DNA fragment, Fragments of salmon DNA are broken to yield polydeoxyribonucleotides. The present application also provides a polydeoxyribonucleotide, in which fragments with a length less than or equal to 400 bp account for more than 90%, and fragments with a length greater than or equal to 1000 bp account for less than 2%. The method provided in this application can synthesize salmon sperm DNA in large quantities by introducing a small amount of exogenous protein (Phi DNA polymerase) through synthetic biology technology. In addition, the salmon sperm DNA synthesized in this application is not full-length genomic DNA (genome DNA, gDNA) but a long fragment DNA with a fragment size of 12kb to 100kb; Obtain a large number of PDRN samples of ideal molecular weight.
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priorityDate 2022-04-27-04:00^^<http://www.w3.org/2001/XMLSchema#date>
type http://data.epo.org/linked-data/def/patent/Publication

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