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filingDate 2022-05-24-04:00^^<http://www.w3.org/2001/XMLSchema#date>
inventor http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_6cd08947078b8723e018e4eda46f369a
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publicationDate 2022-09-23-04:00^^<http://www.w3.org/2001/XMLSchema#date>
publicationNumber CN-115097019-A
titleOfInvention A kind of detection method of methotrexate plasma concentration
abstract The invention discloses a method for detecting the plasma concentration of methotrexate, comprising the following steps: S1, adding methanol to normal serum without methotrexate, and collecting the supernatant to obtain A liquid; S2, The magnetic molecularly imprinted polymer is added to methanol for vibration activation, and the reactant is obtained by magnetic separation; S3, the A solution obtained in step S1 and the reactant obtained in step S2 are respectively added to multiple groups of different concentrations of methotrexate In the standard solution, obtain multiple groups of mixtures, extract and load samples, magnetically separate, and then add an eluent to shake and remove impurities to obtain multiple groups of processed materials; S4. Add the eluent to the multiple groups of materials obtained in step S3 respectively. , after magnetic separation, the supernatant was collected and filtered, and the filtrates were detected by HPLC-UV method respectively to obtain a standard curve; S5, the methotrexate standard solution was replaced with an actual blood sample, and the actual blood sample was obtained by calculation. Methotrexate concentration in blood samples.
priorityDate 2022-05-24-04:00^^<http://www.w3.org/2001/XMLSchema#date>
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