abstract |
The invention discloses a method for detecting PCV3 by using TB Green 1 qPCR, and belongs to the field of biotechnology. The invention discloses a method for detecting PCV3 by using TB GreenПqPCR. By comparing the whole genome sequence, the conservative region of PCV3 is determined, and then specific primers are designed to establish a TBGreenПqPCR detection method. The method of the invention has high sensitivity, good specificity and repeatability, can complete sample detection in a short time, and is suitable for the detection of PCV3 infection in clinical samples, epidemiological monitoring, laboratory research and the like. |