http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-115074428-A
Outgoing Links
| Predicate | Object |
|---|---|
| assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_51c7ef11de093fb283af77dc8a7b8f1b |
| classificationCPCAdditional | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q2600-166 |
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-6806 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-6876 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-6806 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-11 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-6876 |
| filingDate | 2022-04-02-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_b4d9e6b050951b901bf7a4d70e411878 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_94d35229cfbd523bd836ecf98ed24db6 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_64ba29cf060890f5fd2bce7f0f0b87a7 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_58246792b93f3cb84e1c6ca4c6dc8e53 |
| publicationDate | 2022-09-20-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | CN-115074428-A |
| titleOfInvention | Amplification primer, molecular weight internal standard and preparation method for enhancing fluorescence intensity |
| abstract | The invention relates to the field of biotechnology, in particular to an amplification primer for enhancing fluorescence intensity, a molecular weight internal standard and a preparation method. Compared with the prior art, the present invention uses fluorescence resonance energy transfer technology to react the amplification primers labeled with two different excitation wavelengths of fluorescent groups with nucleic acid fragments of a specific length located on the pUC57 plasmid to obtain a uniformly distributed molecular weight internal standard. , which can completely consume the added amplification primers, and is suitable for capillary electrophoresis. The fluorescence signal is more than 10 times higher than the ordinary molecular weight internal standard, which greatly reduces the cost of using the molecular weight internal standard. |
| priorityDate | 2022-04-02-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 58.