| abstract |
The invention discloses a rice blast inoculation identification method. First, outdoor seedlings are raised, and when the seedlings grow to the 3-4 leaf stage, they are placed in an indoor inoculation bucket. A humidifier, an air conditioner and a lighting system are installed indoors, which can adjust the temperature. , humidity and light are strictly controlled. After inoculation with M. oryzae, strictly shade from light, and incubate for 24 hours at 26°C-28°C moisturizing (RH=95%); remove the sealing film and rain cloth, and store in a high-humidity environment at room temperature of 25°C-30°C and RH>95% Cultivated under low temperature, observe symptoms after 5-7 days. This indoor resistance identification method not only avoids the influence of the identification season, but also saves human and material resources, and can truly reflect the resistance and susceptibility of rice varieties. The test results can be used for resistance identification of rice varieties, resistance genetic separation and other research Provide stable and reliable experimental guarantee. |