Predicate |
Object |
assignee |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_b0f54826f671babc7c6de99f2a8f17c5 |
classificationCPCAdditional |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N2800-22 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N2710-12051 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N2469-20 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N2333-01 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/A61K2039-552 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N2710-12022 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N2710-12034 |
classificationCPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07K14-005 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/A61K39-12 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N33-6854 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-70 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N33-56983 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/A61P31-20 |
classificationIPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C07K14-01 |
filingDate |
2021-03-15-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
inventor |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_33951dad238503273ca9e1884aa98648 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_04ddb355006dcb082b476f49cac5692b http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_5a1e35bf39a4801e7a583410b27f3e75 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_d715d53c9d77c6683a1f860a693a1ade http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_9069b666ca78ffa9f410f8ed8e34e76b http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_0d7360ba3c311d82aa6dd1f25e35edde |
publicationDate |
2022-09-20-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
publicationNumber |
CN-115073559-A |
titleOfInvention |
Prokaryotic soluble expression method and application of recombinant African swine fever virus EP153R subunit transmembrane protein |
abstract |
The invention discloses a prokaryotic soluble expression method and application of a recombinant African swine fever virus EP153R subunit transmembrane protein, the amino acid sequence of which is shown in SEQ ID NO. Bacillus codon bias characteristics, artificial optimization and synthesis of African swine fever virus EP153R protein coding gene, the amino acid sequence is shown in SEQ ID NO.3, the gene sequence is shown in SEQ ID NO.1; 2) The codon The optimized gene of African swine fever virus EP153R protein is cloned into a prokaryotic expression vector as shown in SEQ ID NO. 1 to obtain a recombinant plasmid containing the gene encoding the African swine fever virus EP153R subunit protein; Unit transmembrane protein expression and purification. The present invention can provide an African swine fever transmembrane protein EP153R subunit protein that can be industrially produced on a large scale, and the preparation method is simple and low in cost, and can meet the existing national standards. |
isCitedBy |
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-115261395-A http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-115261395-B |
priorityDate |
2021-03-15-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
type |
http://data.epo.org/linked-data/def/patent/Publication |