| abstract |
The invention discloses an enzyme-linked immunization kit for detecting tobacco root black rot fungus and its application. The hyphae and spores of tobacco root black rot are obtained by culturing, immunizing mice with the hyphae and spores, and screening to obtain Specific monoclonal antibodies, and finally developed an enzyme-linked immunosorbent quantitative detection kit for quantitative detection of tobacco root black rot bacteria. It includes: obtaining the hyphae and spores of Tobacco root black rot fungus by culturing; immunizing 6-week-old female BALB/c mice with the hyphae and spores, and then fusing the spleen cells of the immunized mice with myeloma cells SP2/0, and screening The specific monoclonal antibody cell line is obtained, and the specific monoclonal antibody is obtained by purification by preparing ascites; HRP-conjugated antibody is used, and the paired antibody is screened by the ELISA method of double antibody sandwich to obtain an ELISA kit system. The method can qualitatively and quantitatively detect the black rot fungus of tobacco roots in tobacco, and has the advantages of strong specificity, high sensitivity, simplicity and rapidity. |