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filingDate 2022-06-06-04:00^^<http://www.w3.org/2001/XMLSchema#date>
inventor http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_e3c9f704f8103e75a729c7f3cbb8d0fc
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publicationDate 2022-09-16-04:00^^<http://www.w3.org/2001/XMLSchema#date>
publicationNumber CN-115060894-A
titleOfInvention A kind of label-free electrochemical immunosensor and its preparation method and application
abstract The invention discloses a label-free electrochemical immunosensor and a preparation method and application thereof. The method comprises: mixing graphene oxide, L-cysteine and boric acid, adding deionized water to disperse evenly by ultrasonic wave, and adjusting pH To 8 to 9, perform hydrothermal reaction to obtain boron, sulfur, nitrogen co-doped reduced graphene oxide; obtain a gold nanoparticle solution; disperse the boron, sulfur, nitrogen co-doped reduced graphene oxide in the gold nanoparticle In the particle solution, washed and dried to obtain Au-B,S,N-rGO nanocomposite; EDC and NHS solution were added for activation and then drop-coated on the surface of glassy carbon electrode to obtain Au-B,S,N-rGO electrode ; drop an antibody solution on the surface of the electrode for washing after incubation, then dropwise add bovine serum albumin for washing, and then dropwise add a standard antigen solution to obtain a label-free electrochemical immunosensor. The label-free electrochemical immunosensor has low detection limit, high sensitivity and high specificity.
priorityDate 2022-06-06-04:00^^<http://www.w3.org/2001/XMLSchema#date>
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