http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-115058488-A
Outgoing Links
| Predicate | Object |
|---|---|
| assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_0677b252fc36aca0c2cf53c0cc336ca4 |
| classificationCPCAdditional | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q2600-178 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q2600-158 |
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-6844 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-6886 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-6804 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-6886 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-6804 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-6844 |
| filingDate | 2022-07-27-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_f5f1b807404b49fdf52d17fd05908c8c http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_1e503b8bb4411343c77533c76c294e2d http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_11ee7e18196eb244346c10d8787428f2 |
| publicationDate | 2022-09-16-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | CN-115058488-A |
| titleOfInvention | Visual detection method of circular RNA based on exosome extraction from peripheral blood |
| abstract | The invention discloses a circRNA visualization detection method based on the extraction of exosomes in peripheral blood, which can stably and efficiently extract and purify exosomes from peripheral blood, and can detect pancreatic cancer-specific circRNAs with high sensitivity, thereby realizing the detection of pancreatic cancer. One-stop detection solution, specifically, the present invention uses polymer precipitation combined with chromatography to solve the problem of low extraction efficiency of exosomes in peripheral blood, realizes stable and efficient purification of exosomes, and uses reverse transcription-rolling Ring amplification reaction (RT-RCA), in the present invention, the amplification substrate is A base containing biotin label, so that the amplified product can be detected by streptavidin colloidal gold test strip, and the present invention gets rid of expensive The dependence on instruments (such as ultracentrifuges, fluorescence quantitative PCR instruments, etc.) and precise operation requirements simplifies the detection methods of pancreatic cancer, improves the sensitivity, and finally facilitates the formation of commercial kits and improves product competitiveness. |
| priorityDate | 2022-07-27-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 33.