| Predicate |
Object |
| assignee |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_5aa816a5ff9b10cd3550a416ce9f8e0b |
| classificationCPCAdditional |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/A01K2267-0356
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/A01K2227-40
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/Y02A40-81
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N2310-20 |
| classificationCPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-113
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07K14-461
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/A01K67-0275
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/A61K49-0008
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N9-22
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-89 |
| classificationIPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-113
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-12
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N9-22
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/A01K67-027
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/A61K49-00
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-89 |
| filingDate |
2022-06-28-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_d31603b7cd4e5a22e619be1632d0fdfe
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_d3ce3ec29db54aced2e333ba124b441d
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_2f6632f06c36e79695218e7a503e49ab |
| publicationDate |
2022-09-16-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber |
CN-115058424-A |
| titleOfInvention |
An irf2bpl knockout zebrafish epilepsy model and its construction method and application |
| abstract |
The present invention provides a sgRNA combination for CRISPANT to specifically knock out irf2bpl gene in zebrafish to construct a zebrafish epilepsy model, the sequences are shown in SEQ ID NO.1, SEQ ID NO.3 and SEQ ID NO.4. In addition to the 7bp fragment on exon 1 of the zebrafish irf2bpl gene, the irf2bpl gene deletion zebrafish was selected to construct the zebrafish epilepsy model. The invention uses three sgRNAs to target the same gene, maximizes the editing efficiency, and performs gene editing more effectively. The present invention can more efficiently and accurately knock out a specific gene in the genome of an organism, and has the advantages of simple manufacture and low cost. The zebrafish epilepsy model provided by the present invention lays a good foundation for further research on the relationship between irf2bpl mutation and epilepsy pathogenesis and screening of antiepileptic drugs. |
| isCitedBy |
http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-114958857-A |
| priorityDate |
2022-06-28-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type |
http://data.epo.org/linked-data/def/patent/Publication |