http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-115046973-A
Outgoing Links
| Predicate | Object |
|---|---|
| assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_d6a6f422b091ba12ea61d4adbf1b0e8e |
| classificationCPCAdditional | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07K2319-00 |
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N9-50 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N21-6486 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07K14-43595 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07K14-4703 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N9-50 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N21-64 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C07K19-00 |
| filingDate | 2022-06-12-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_258690b3b9383e8105d337de8aad089a http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_96abd9864e8bfdae897581b408b98c2e http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_c53a0581f571efff6acc2c26efd17337 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_41cfe84341dc333b830207488612b2c4 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_02487787f318c6c275aa9043e3142d28 |
| publicationDate | 2022-09-13-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | CN-115046973-A |
| titleOfInvention | DNA damage detection method and functional protein based on FRET |
| abstract | The invention discloses a DNA damage detection method and functional protein based on FRET. Using the repressor protein DdrO in Deinococcus radiodurans, the yellow fluorescent protein eYFP and the cyan fluorescent protein eCFP were linked to its N-terminus and C-terminus, respectively, to construct a functional protein YDC with fluorescence energy transfer properties; Deinococcus radiodurans damage response regulator PprI protein, the protein PprI‑D91A was obtained by mutating the aspartic acid (D) at position 91 of the PprI protein to alanine (A) to reduce the absence of single chain DNA enzyme cleavage activity; PprI‑D91A can rapidly digest YDC when single-stranded DNA produced by damage exists in the system. Under the action of 440 nm excitation light, the 480 nm peak of YDC emission light increases and 530 nm The peak decreased; the amino acid sequences of YDC and PprI‑D91A and the reaction system for detecting DNA damage are also provided. The present invention has important guiding significance for developing new molecular biology tools. |
| priorityDate | 2022-06-12-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 28.