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filingDate 2022-06-30-04:00^^<http://www.w3.org/2001/XMLSchema#date>
inventor http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_970201ca68fc74280ae4a40947d88639
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publicationDate 2022-09-13-04:00^^<http://www.w3.org/2001/XMLSchema#date>
publicationNumber CN-115044701-A
titleOfInvention Specific probe, quantitative detection method and application of Saccharomyces cerevisiae
abstract The invention belongs to the technical field of liquor brewing, and particularly relates to a specific probe, quantitative detection method and application of Saccharomyces cerevisiae. The technical problem solved by the present invention is to provide a specific probe, quantitative detection method and application for quickly and accurately identifying Saccharomyces cerevisiae. The method of the present invention includes the following steps: mixing the sample to be tested with the hybridization chain reaction probe buffer and reacting fully, then adding the substrate hairpin probe to react, and then adding hemin, ABTS and hydrogen peroxide to mix reaction, and quantify the Saccharomyces cerevisiae in the sample to be tested according to the final color of the mixed solution. The invention realizes the identification, signal amplification and final colorimetric detection of the 26s rDNA of Saccharomyces cerevisiae by designing a probe detection system, and realizes the ultra-sensitive and high sensitivity of the 26s rDNA of Saccharomyces cerevisiae by constructing an ingenious and streamlined isothermal amplification system. Specific and simple portable colorimetric assay.
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Total number of triples: 28.