abstract |
The invention relates to the application of cyp19a1b as a biomarker for combined exposure to tritium water and genistein, and relates to the technical field of genetic engineering and detection. In the present invention, the zebrafish embryos are cultured in a culture medium, and are divided into blank control group, tritium water group, lignoflavonoid group, and tritium water and lignoflavonoid combination groups; and then the growth process from zebrafish embryos to zebrafish larvae is observed, The survival indicators of zebrafish embryos and zebrafish larvae were recorded; finally, the zebrafish in the blank control group and the tritium water and genistein combination group at 96hpf were analyzed to screen out the tritium water and genistein combined exposure biomarkers. |