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filingDate 2022-07-04-04:00^^<http://www.w3.org/2001/XMLSchema#date>
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publicationDate 2022-09-09-04:00^^<http://www.w3.org/2001/XMLSchema#date>
publicationNumber CN-115032406-A
titleOfInvention Detection reagent and preparation method and application thereof
abstract The present invention relates to the field of biological detection, in particular to a detection reagent and a preparation method and application thereof. The present invention provides detection reagents, including: biotinylated-mugwort allergen extract; the above-mentioned biotinylated-mugwort allergen extract includes: mugwort allergen extract and biotin; the above-mentioned mugwort allergen extract Including: mugwort extract and mugwort phytodefensin-like protein. The detection reagent of the present invention is prepared by adding a natural mugwort allergy protein phytodefensin-like protein to the mugwort extract, and carrying out biotinylation labeling according to a specific molar ratio to prepare a biotinylated- mugwort allergen extract solution, which can improve the performance of the detection reagent. Sensitivity and specificity of detection. At the same time, the detection reagent is suitable for an automatic magnetic particle chemiluminescence instrument, and the reaction time is short, the operation is simple and the degree of automation is high.
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