| Predicate |
Object |
| assignee |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_29ce560b2cad4652b3d77e980e87699f |
| classificationCPCAdditional |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N2015-1006
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N2509-00
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N2509-10
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N2015-0065 |
| classificationCPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N33-5047
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N5-0634
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N33-534
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N33-60
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N15-1429
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N33-6854 |
| classificationIPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N33-534
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N33-50
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N33-68
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N33-60
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N15-14
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N5-078 |
| filingDate |
2021-11-26-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_bde36280e714e18edf5f9b44a6ef6926
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_3f21d7865c3611a4418e5ea3fb5d9357
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_c8bb772b0697cf9e19829b1ef3895eed
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_d5e1bc6cf72afa9fdc9c8abf5dc9f50a |
| publicationDate |
2022-09-09-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber |
CN-115032374-A |
| titleOfInvention |
Methods for establishing the characteristics of lung immune cells in mice with acute lung injury induced by snake venom |
| abstract |
The invention provides a method for establishing the characteristics of lung immune cells in mice with acute lung injury caused by snake venom, comprising the following steps: S1 extracting immune cells; S2 coupling; S3 staining; S4 biosignal analysis; the beneficial effects of the present invention: lung The tissue was evenly shredded for dissociation, and centrifuged according to the cell density to obtain a large number of immune cells, which were washed with high-glucose medium to maximize the yield and activity of immune cells in the lungs; it can be obtained by trypan blue staining. The viability of the obtained cells is basically greater than 90%; 42 antibodies can be detected simultaneously on mouse lung immune cells, cell-specific markers and functional proteins can be detected, and the dynamic changes of lung cell subsets can be observed. |
| priorityDate |
2021-11-26-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type |
http://data.epo.org/linked-data/def/patent/Publication |