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filingDate 2022-04-25-04:00^^<http://www.w3.org/2001/XMLSchema#date>
inventor http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_b6ef0c2b5bb3ccdd8d456f8937384af1
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publicationDate 2022-09-09-04:00^^<http://www.w3.org/2001/XMLSchema#date>
publicationNumber CN-115029436-A
titleOfInvention A PCR kit for detecting RET gene M918T mutation and its detection method
abstract The invention relates to the field of gene mutation detection, and discloses a PCR kit for detecting RET gene M918T mutation, including nucleic acid amplification reagents and reference substances. The nucleic acid amplification reagent includes RET M918T reaction solution, which contains specific primers and fluorescent probes for detecting RET gene M918T mutation. The nucleic acid amplification reagent also includes ddPCR Mix. The invention also discloses a detection method of the above-mentioned detection method of the PCR kit for detecting the M918T mutation of the RET gene. The kit of the present application adopts digital PCR technology, which can quantitatively detect the M918T mutation of the RET gene in fine needle aspiration biopsy samples of thyroid nodules, thereby assisting in the diagnosis of benign and malignant thyroid nodules.
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