| abstract |
The present invention relates to the field of tumor biotherapy, in particular to a recombinant oncolytic adenovirus and application thereof. The genome E1 and E3 regions of the recombinant oncolytic adenovirus are deleted, and the deletion regions are stably inserted into the outside of cholesterol regulatory elements and immune regulatory elements. source nucleic acid sequence; wherein, the cholesterol regulatory element is a functional element comprising the APOA1 gene or its degenerate sequence; the immune regulatory element is a functional element comprising a group selected from GM-CSF, IFN-γ, IL2, IL7, IL12 , IL15, IL16, IL18, IL21, IL22, IL27, IL28 and IL29 genes or functional elements including their degenerate sequences; the genes of the cholesterol regulatory elements and immune regulatory elements are expressed in a non-fusion manner; the cholesterol Regulatory and immunomodulatory elements are operably linked to exogenous regulatory sequences, including promoter sequences, enhancer sequences, and PA sequences. The functional elements carried by the recombinant oncolytic virus can synergistically stabilize the T cell function in the tumor microenvironment. |