abstract |
The invention provides a method for preparing human parathyroid hormone 1-34 by fusion expression. A microbial expression system is used to express a fusion protein, and the fusion protein includes glucagon-like peptide and human parathyroid hormone 1-34 from N-terminal to C-terminal, wherein the glucagon-like peptide includes glucagon-like peptide One or more of peptide-1 and its analogs, glucagon-like peptide-2 and its analogs. In the present invention, GLP protein is used to promote the expression of PTH1-34, and at the same time, the proportion of parathyroid hormone 1-34 in the expression frame is increased, so that the proportion of PTH1-34 in the fusion protein is not less than 60%, which greatly improves PTH1 ‑34 yield. |