| abstract |
The invention discloses a new application of a small molecular peptide, namely the application of a horse milk casein small molecular peptide in the preparation of a dipeptidyl peptidase IV inhibitor, wherein the amino acid sequence of the small molecular peptide is Pro-Gln-Pro-Ile ‑Glu‑Arg‑Thr, the molecular weight is 839.95 Da; the small molecule peptide of the present invention has dipeptidyl peptidase IV inhibitory activity, the inhibition rate of dipeptidyl peptidase IV is 86%, and the half inhibitory concentration (IC 50 ) is 0.726 mg/mL; and can improve the insulin resistance of high glucose-induced (human liver cancer) Hepg2 cells, significantly increase the activity of Hepg2 cells, and improve the glucose consumption and glucose uptake of Hepg2 cells; the small molecular peptides of the present invention can be used in the preparation of therapy or adjuvant therapy 2 The functional food, health care product and medicine for type diabetes mellitus are simple to prepare and suitable for industrial production and market promotion and application. |