| Predicate |
Object |
| assignee |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_b06ba1ea64c76a3fbce6b23053ac174b |
| classificationCPCAdditional |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12R2001-19
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/Y02A50-30 |
| classificationCPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07K14-465
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-70
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12P21-02 |
| classificationIPCAdditional |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12R1-19 |
| classificationIPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-70
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C07K14-465
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12P21-02
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C07K1-36
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C07K1-30
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C07K1-34
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N1-21
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C07K1-22 |
| filingDate |
2022-05-09-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_d63947c7364a02b0fcc2561cfafc6423
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_35498a3153a0a8077476af9e578b48a5
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_c60a1c4c4979cd0c9483f0d569ffd569
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_5b464d4534a98fd837955cddc83d5410
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_a2e502c8a875a1b4bdad3b7d463bdda7 |
| publicationDate |
2022-09-09-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber |
CN-115028705-A |
| titleOfInvention |
HNF6 polypeptide fragment and its expression and purification method |
| abstract |
The invention discloses an HNF6 polypeptide fragment and an expression and purification method thereof, belonging to the field of biotechnology. The present invention firstly optimizes and synthesizes chicken Hnf6 gene according to the codon preference of Escherichia coli, and constructs a prokaryotic recombinant expression vector pET-30a(+)-Hnf6. Secondly, the recombinant expression vector was transformed into Escherichia coli BL21 (DE3) for induced expression, 6 mol/L urea denaturation inclusion bodies were used to dissolve the recombinant protein, and the 6-His polypeptide fused to the C-terminus of the HNF6 recombinant protein was used for Ni 2+ to dissolve the recombinant protein. The recombinant protein was purified by affinity chromatography, and the target protein was renatured with the rapid renaturation method. The results showed that a high-purity recombinant protein HNF6 was successfully obtained from the inclusion bodies by a one-step purification method that combined Ni 2+ affinity chromatography and in situ renaturation of the target protein, which provided research on the preparation of chicken HNF6 antibodies. material security. |
| priorityDate |
2022-05-09-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type |
http://data.epo.org/linked-data/def/patent/Publication |