http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-115023505-A
Outgoing Links
| Predicate | Object |
|---|---|
| assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_71eecf8043d657dbc5967938c3eebc09 |
| classificationCPCAdditional | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q2600-16 |
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-70 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-6876 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-70 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-6876 |
| filingDate | 2021-10-19-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_32dcc8d3330939ef4224b7feb69c7046 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_29451227847772d4833921c75da7a089 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_cc268e1a78b4982182d142870541b39f http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_b9c2a76439b8890082c089d20729824f |
| publicationDate | 2022-09-06-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | CN-115023505-A |
| titleOfInvention | Parallel nucleic acid analysis method |
| abstract | The present invention provides a method of detecting target nucleic acids in a plurality of samples, the method comprising the steps of: a) providing analyte nucleic acids from the plurality of samples in separate containers for each sample, wherein the containers are arranged to an array of subsets, wherein the array includes two or more subsets; b) amplifying nucleic acids by a primer extension reaction using at least one pair of primer pairs that hybridize to the analyte nucleic acid, wherein the primer pairs comprise a forward primer and a reverse primer forward primers, wherein the forward and reverse primers each comprise an adaptor sequence, a sample identifier sequence, and a binding sequence for hybridization to the analyte nucleic acid; c) combining two or more subsets of containers The amplified nucleic acids of step b) are combined into an array of combined containers, wherein the containers of one subset, but not the other subset, are combined into a combined container; d) hybridization using said amplified nucleic acids of the combined containers of step c) is used at least one pair of additional primer pairs amplifies nucleic acid by a primer extension reaction, wherein the additional primer pair comprises an additional forward primer and an additional reverse primer, wherein the additional forward primer and the additional reverse primer both comprise A subset identifier sequence and a sequence for hybridization with said adapter sequence; e) determining the sequence of the nucleic acid amplified in step d); f) by combining with said subset identifier sequence and said sample identifier sequence The subset is associated with the container, assigning the target nucleic acid sequence determined in step e) to the sample. Primer sets for this method are also provided. |
| priorityDate | 2020-10-19-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 161.