http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-115015449-A

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filingDate 2022-05-23-04:00^^<http://www.w3.org/2001/XMLSchema#date>
inventor http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_79eb462f8d57b9b0d18a4383c6218975
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publicationDate 2022-09-06-04:00^^<http://www.w3.org/2001/XMLSchema#date>
publicationNumber CN-115015449-A
titleOfInvention A kind of concentration process of effective components in toad venom
abstract The invention discloses a process for concentrating effective components in toad venom, comprising the following operation steps: S1: preparation of a test sample: 10 mL of methanol is used for the standard samples (20 mg each) of bufalin, leopufactin and cinobufinoside, respectively. Dissolved as a standard at a concentration of 2 mg/mL, the three standards were formulated into 1 mg/mL, 0.8 mg/mL, 0.6 mg/mL, 0.4 mg/mL, 0.2 mg/mL, 0.1 mg/mL, 0.05 mg/mL S2: Take about 2g of the test sample, lay it flat in a flat glass weighing bottle dried to constant weight, the thickness should not exceed 5mm, and the loose test sample should not exceed 10mm, and accurately weighed. The method for concentrating the effective components in toad venom according to the present invention is to conveniently measure the content thereof by concentrating the effective components bufalin, cinnabufoxin-based and bufatoxin in the toad venom.
priorityDate 2022-05-23-04:00^^<http://www.w3.org/2001/XMLSchema#date>
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