| abstract |
The invention discloses a tartary buckwheat active peptide, a preparation method and a new use thereof, namely the application of the tartary buckwheat active peptide in medicines, food and health food for preventing and treating alcoholic liver disease. The peptide sequence of the tartary buckwheat active peptide is Lys‑Leu‑Arg‑Pro‑Leu‑Gln‑Val‑Leu, and the preparation method includes: using tartary buckwheat as a raw material, undergoing solid-state fermentation with Aspergillus sojae and ultrasonic treatment to obtain a crude solution of the tartary buckwheat active peptide , followed by ultrafiltration treatment, Sephadex column separation and high-performance liquid chromatography column purification, sequence identification by Q‑Exactive LC‑MS/MS, and preliminary screening. After screening, the peptides were docked with alcohol oxidase molecules again After screening and synthesis, the peptide with the best activity was selected after the in vitro antioxidative and anti-inflammatory activity was measured. Through research, it is found that the tartary buckwheat active peptide is used for the treatment of alcoholic liver disease. |