| abstract |
The invention discloses a preparation method of a bivalent dysentery conjugate combination vaccine, which belongs to the technical field of biological product preparation. The vaccine includes two serotypes of flexneri 2a and sonnei. The O-specific polysaccharides of the two bacteria are covalently combined with carrier proteins according to their unique chemical structures to prepare a stock solution of polysaccharide-protein conjugates by corresponding chemical methods. , the polysaccharide protein conjugate stock solution is then adsorbed with aluminum adjuvant according to an appropriate ratio to obtain dysentery conjugate vaccine. Among them, the Fuchs 2a dysentery polysaccharide is prepared by the reductive amine method, and the Sonnet's dysentery polysaccharide is prepared by the direct combination method with a spacer. In particular, the present invention no longer uses highly toxic reagent cyanogen bromide and high activation pH, the method has no toxic reagent, mild reaction, easy control, and is beneficial to large-scale production in the future. The dysentery conjugate vaccine prepared by the invention is used for immune prevention of related serotype dysentery bacillus infection. |