http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-114994310-A
Outgoing Links
| Predicate | Object |
|---|---|
| assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_00d27136cec99c04ab41cdb69cbacc7c |
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N33-48721 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N33-54326 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N33-543 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N33-487 |
| filingDate | 2022-05-26-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_2b382cfa8bce5a3f684c6cc89f2f4d99 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_f91f16af747707210f449f8bdef59314 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_90174f59f409cd0c52785a55d23abf4d http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_96875d5b5446a6ba6636214a14caf66e http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_64d36524fe8d586499272a484528d406 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_419f560b168d6346ba023cec87484998 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_99b3df32177a2ad487dd427699a453ed |
| publicationDate | 2022-09-02-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | CN-114994310-A |
| titleOfInvention | A biological nanopore detection method based on 3D DNA walker sensing and its application in MUC1 detection |
| abstract | The invention provides a biological nanopore detection method based on 3D DNA walker sensing and its application in MUC1 detection. The detection method comprises: hybridizing the Zn 2+ -DNAzyme walking chain with terminal biotin and the aptamer to be tested, and then fixing it and the biotin-modified hairpin substrate chain on Fe 3 O 4 particles respectively. In the above, when the analyte specifically interacts with the aptamer of the analyte, Zn 2+ -DNAzyme is released, and the DNAzyme cleaves the hairpin substrate strand in the presence of Zn 2+ , releasing the substrate strand DNA fragment; After the Fe 3 O 4 particles were separated, the released DNA fragments were detected by the α-HL biological nanopore to obtain the concentration of the analyte. The biological nanopore detection method based on 3D DNA walker sensing provided by the present invention utilizes the characteristics of the 3D DNA Walker nanoprobe to amplify the signal, and combined with the biological nanopore detection, the sensitivity and signal-to-noise ratio of the nanopore detection can be greatly improved, The lowest detection level of MUC1 can reach 0.01nM. |
| priorityDate | 2022-05-26-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 40.