| abstract |
The invention discloses a feasibility assessment method based on total fecal DNA for different genetic analysis, belonging to the technical field of molecular biology. In order to fill the gaps in the quality control methods and feasibility standards of the current fecal DNA application in different genetic analysis scenarios, the present invention targets the 16srRNA gene of bacteria, the ND5 gene on the animal mitochondrial genome, and the RPS27A gene on the nuclear genome, and adopts corresponding The primers were used to establish a quantitative PCR system to determine the copy numbers of bacterial DNA, animal mitochondrial DNA (mtDNA) and nuclear DNA (nuDNA) in total fecal DNA. Taking the copy number of bacterial DNA as a reference, two indicators of relative abundance of mtDNA and nuDNA (R mtDNA , R nuDNA ) were constructed, and fecal DNA was established for PCR-based SNP typing, STR typing and A model of the relationship between success rates and R mtDNA and R nuDNA in high-throughput sequencing-based mitochondrial genome assembly and whole-genome resequencing scenarios as a tool to pre-assess the usability of fecal DNA in different genetic analyses. |