| abstract |
The invention discloses an improved method for separating and culturing mouse ovarian germline stem cells, which comprises the steps of taking ovaries, separating cells by two-step enzymatic method, primary culture and passage of female germline stem cells, and cryopreservation of female germline stem cells. The pre-digestion treatment, the digestion time of trypsin and collagenase are improved, and a simple and efficient method for separating ovarian germline stem cells is obtained. The invention improves the separation and culture method system of mouse ovarian germline stem cells. , The time of two-step enzyme treatment, purification method, and culture method have been improved, and mouse ovarian germline stem cells have been successfully isolated and cultured in vitro, and can be stably passaged. Research in the field of stem cells is of great significance. The method of the present invention is simple and clear, requires low experimental conditions, and is highly operable. |