| abstract |
The invention discloses a tumor organoid chip for multi-cell interaction and drug screening and a preparation method thereof. The tumor organoid chip comprises a hard base material layer and a PDMS layer disposed above the hard base material layer; wherein , the interior of the PDMS layer includes a fluid inlet and a fluid outlet, a fluid channel connected with the fluid inlet and the fluid outlet, and three culture chambers surrounded by the fluid channel, and the periphery of the three culture chambers is composed of a micro-pillar array; the present invention The micro-column array is used to form a relatively independent perfusion and culture space, and the same dynamic fluid culture conditions can be given to the cells in the chamber, which can not only study the interaction of different cells, but also serve as a In the cultivation of the same kind of cells, the chip of the present invention can observe the occurrence, growth and metastasis of tumors in real time, so as to accurately evaluate the actual effect of drugs in different stages, and has a high application prospect for the future personalized precision tumor treatment. |