| abstract |
The present invention discloses a 3G9-LNM antibody-coupled protein and a preparation method and application thereof. The protein is obtained by combining a tumor antigen and an antibody by breaking intein trans-splicing. The amino acid sequence of the protein heavy chain is shown in SEQ ID NO: 10, The amino acid sequence of the light chain is shown in SEQ ID NO: 6, and the light chain does not participate in the reaction. The 3G9-LNM antibody-coupled protein provided by the present invention, as well as the preparation method and application thereof, overcome the problems such as the need to add chemical reagents, certain toxicity, unstable chemical coupling, and easy breakage of coupling bonds in the existing antigen-antibody coupling method, The connection using the split intein GP41-1 trans-splicing method is connected by peptide bonds, which has higher stability, stronger specific binding, mild reaction conditions, high efficiency, simple process, and no toxic and harmful substances are added. It will not produce by-products, etc., with high safety, and provide technical support and new ideas for the research and development of new tumor vaccines. |