| abstract |
The invention relates to the field of extraction and separation of natural medicines, and discloses a method for preparing natural sphingomyelin, comprising the following steps: A. obtaining crude sphingomyelin; B. dissolving the crude sphingomyelin in an alcohol-ether-water ternary mixed solution In the process, recrystallization is carried out to obtain a sphingomyelin product; the alcohol-ether-water ternary mixed solution includes short-chain alcohol, ether solvent and water. The technical scheme of the present invention uses egg yolk-derived phospholipids as raw materials, first obtains sphingomyelin with a mass ratio of more than 30% through column chromatography, and then uses a solvent of a ternary mixed system to recrystallize sphingomyelin, and after drying, the obtained sphingomyelin with a purity higher than 30% is obtained. 98% natural sphingomyelin. The method has the advantages of simple preparation, less solvent consumption and high refining yield, which greatly reduces the production cost and is easy for industrialized production. |