http://rdf.ncbi.nlm.nih.gov/pubchem/patent/CN-114959919-A
Outgoing Links
| Predicate | Object |
|---|---|
| assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_4d9dbc2c0705931153800d351be59f09 |
| classificationCPCAdditional | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/Y02E50-10 |
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C40B40-02 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07K14-395 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C40B30-06 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-81 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C40B50-06 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-81 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C40B50-06 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C40B40-02 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C40B30-06 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-113 |
| filingDate | 2021-02-23-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_ca4ed57ee915e179124e30e94eb458f5 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_15176c897af2b335c28dc5f9580a8609 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_bbc33346be521ff5e974d794c5115339 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_fc2f516dd38d9bdcf5d7f967cf191956 |
| publicationDate | 2022-08-30-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | CN-114959919-A |
| titleOfInvention | A method and application of constructing Saccharomyces cerevisiae artificial small promoter library |
| abstract | The invention discloses a method and application for constructing a small artificial promoter library of Saccharomyces cerevisiae. The present invention provides a method for constructing a library of Saccharomyces cerevisiae artificial promoter mutants, comprising: the nucleotide sequence of Saccharomyces cerevisiae artificial promoter UASF-E-C-core1 in the 1-6 position from the 3' end All or part of the saccharomyces cerevisiae artificial promoter mutant library is obtained by random mutation. In the present invention, the strongest small synthetic promoter obtained by Alper is used as a scaffold, and green fluorescent protein (GFP) is used as a reporter gene to construct a kozak library. And apply it to the metabolic regulation of the endogenous pathway of Saccharomyces cerevisiae, so that the genes in the metabolic pathway can match the appropriate expression level, and avoid the accumulation of toxic intermediate metabolites, which will inhibit cell growth or cause the output of target products due to insufficient expression intensity of key genes. lower issues. |
| priorityDate | 2021-02-23-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 43.