| abstract |
The invention relates to the technical field of genetic engineering, and discloses a detection method for rapid detection of GAstV based on MIRA fluorescence method, comprising the following steps: S1, processing of tissue samples: weighing 1 g of each tissue sample, adding 1 mL of PBS, and putting it into grinding Grind into a homogenate in a bowl, transfer to a sterile centrifuge tube, and centrifuge at 8500rpm/min for 3min in a centrifuge; S2, extraction of viral nucleic acid: use a viral genome DNA/RNA extraction kit (centrifugation) from a biochemical technology company. Column type) to extract viral RNA; specific primers and probes are designed in the conserved region of the GAstV ORF2 gene of the present invention, and MIRA technology is used to amplify the nucleic acid of GAstV, and a rapid detection method of GAstV is established. The method improves the biological detection efficiency, lowers the detection threshold, saves detection time, and is simple to operate. It does not require high equipment and can obtain results within 20 minutes, and can monitor epidemic diseases in real time even in remote areas. . |