| abstract |
A multiplex PCR detection method for gene expression analysis of PWS imprinted region belongs to the technical field of molecular biology. The method is used for expression analysis of the MAGEL2 gene in the genome, and furthermore, the imprinted state of the PWS imprinted region in the chromosome 15q11.2-q13 region is analyzed. Evaluation for quick, easy and accurate results. The detection method adopted in the present invention has the advantages of rapidity, high throughput, less required sample volume, low price and simple operation, and the required instruments and equipment are commonly used in laboratories, which are easy to be carried out in clinical laboratories. |